Clinical/Forensics Instruments

Gilson 333/334 Preperative Scale HPLC Pumps

Gilson Prep-Scale HPLC Pumps

Your delivery solution for preparative chromatography. Capable of fast separations with high-efficiency columns in normal and reverse-phase modes. Choose the 333 Master Pump for isocratic applications or a 333/334 combination for binary gradient applications.

  • Milligram-to-gram level sample mass throughput per injection; up to 15 g depending on column size and loading capacity
  • Composition gradient with high-pressure mixing: 333-334 binary system (third pump for ternary). Solvent selection on each pump with addition of four-solvent valve (up to eight solvents)
  • Ability to operate additional pumps in parallel to double the flow rate up to 400 mL/min
  • Control from 333 master pump: flow-rate gradient, four-solvent selection and step gradient (additional valve), composition gradient (one or two additional pumps), sample-injection pump, UV/VIS detector and electrical input/output contacts with any other associated equipment
  • Quiet operation, durable design
  • Control 33X Pumps and an entire preparative HPLC system via TRILUTION® LC Software
  • Stackable to conserve bench space



Manufacturing Standards Meet applicable Safety and EMC certification standards; UL and CE certified

Composition and Mixing Increment: 0.1%
Dwell volume: 3.7 mL, pressure independent
Gradient linearity range: 1–98% B for 10 mL/min and higher

Environmental Conditions Indoor use
Altitude: up to 2000 m
Temperature range: 5–40°C
Air pressure: 75–105 kPa
Pollution degree: 1 or 2, in accordance with IEC 66
Humidity: Maximum relative humidity 80% for temperatures up to 31°C, decreasing linearly to 50% relative humidity at 40°C

Flow Rate 1–200mL/min (3,040 psi)
Selectable units: L/hr and mL/min
Adjustment increment: 0.01 mL/min
Minimum adjustable value: 20 µL/min
Accuracy: bias <±1% or 5.3 µL/min (333), bias <±2% or 53 µL/min (333/334)
Repeatability: RSD <0.5% or 2.7 µL/min (333), RSD <0.7% or 27 µL/min (333/334)

Front Panel
(333 Pump)
40-character, eight-line display, adjustable contrast and screen saver; 25 functional keys, including six soft keys

Liquid-Contact Materials 316L stainless steel, titanium, FEP, PTFE, PCTFE, ETFE, PEEK, UHMWP, ruby, and ceramics

Power Requirements Frequency: 50–60 Hz
Voltage: 90–120 or 220–260V; mains voltage fluctuations not to exceed ±10% of the nominal voltage

Pressure Nominal range: from 0.5 MPa (5 bar, 70 psi) up to 21 MPa (210 bar, 3,040 psi)

Pump Options 333: single-solvent master pump (w/control panel), 0.2–200 mL/min, 21 MPa (3,040 psi)
333/334: two-solvent pumping system, 2–200 mL/min 21 MPa (3,040 psi)
334: third-solvent, remote controlled pump (w/o control panel), 2–200 mL/min, 21 MPa (3,040 psi)

Software Via TRILUTION® LC Software and 506C interface or GSIOC slave bus

(w x d x h)
333: 26 x 41 x 51 cm (10.2 x 16.2 x 20.1 in.)
334: 26 x 41 x 39 cm (10.2 x 16.2 x 15.3 in.)

Weight 34 kg (75 lbs.)





HPLC Pumps

HPLC Systems


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